In order to investigate the effect of water deficit tension and zeolit application on the amount of damage biomarkers, relative content of leaf water, electrolytes leakage and chlorophyll of rapeseed (Brassica napus L.) Okapi cultivar, the present experiment was done as split plot based on based on randomized complete blocks design with four replications during fall 2010-11 at Islamic Azad University field research , Yadegar-e-Imam Khomein ^(RAH) Shahr-e-Rey Branch. Irrigation at three levels (irrigation based on 80, 130 and 180 millimeter evaporation from class A evaporation pan) as main plot, zeolite consumption in three levels (no consumption, 6 and 12 tons per hectare consumption) as sub-plot were considered. The results of experiment indicated that all traits tested were significantly different regarding to irrigation levels and zeolite application and the effect of experimented treatments interaction was significant only on electrolytes leakage and chlorophylls content. The mean comparison of main effect of irrigation demonstrated that applying irrigation based on 180 millimeter evaporation from class A evaporation pan, established the highest amount of  Malondialdehyde (2.54 nanomoll per milligram protein), Dihydroxyguanosin (17.60 nanomoll per milligram protein), Dityrosine (27.02 nanomoll per milligram Protein) and electrolytes leakage (1.55 millizimence per cm) and lowest relative leaf water content (0/61 percent) and total chlorophyll (4.32 milligram per liter). In severe drought tension conditions 180 millimeter, using 12 tons per hectare zeolit consumption by the improvement of plant moisture status led to prevent drought tension harm effect and reducing leakage electrolytes to 0.99 millizimence per cm  and chlorophyll a+b to 4.75 milligram per liter.