In order to evaluate the effect of chemical and biological fertilizers on crop and physiological characteristics of wheat, pearl cultivar was applied in experimental form in the form of complete randomized randomized blocks with three replications in Nokandeh city of Golestan province. Experimental factors include nitrogen supply sources at four levels of nitrogen (12 g/100 kg seed), Azospirillum (12 g/100 kg seed), urea (200 kg/ ha^-1) and an equal combination of three sources (33% Azotobacter + 33% Azospirillum + 33% urea) and phosphorus-supplying sources at the level of three levels of triple superphosphate (100 kg / ha), fertile phosphate 2 (12g/100 kg seed) and equal combination of two sources (50% triple superphosphate + 50% fertile phosphate 2)) Was with the control.  The results of analysis of variance showed that nitrogen and biological fertilizers had a significant effect on all traits except the weight of one thousand seeds at the level of one percent probability. The results showed that the highest plant height, biological yield, spike length, number of seeds per spike, grain yield, flowering and care protein, chlorophyll b and total fertilizer treatment combined the three sources of nitrogen supply (33% Azotobacter + 33% of Azospirillum + 33% Urea). High levels of leaf area index, spike and chlorophyll a were obtained with Azotobacter treatment. In summary, the results of this study showed that using the right combination of biofertilizers and nitrogen fertilizers can reduce the use of chemical fertilizers and therefore be consistent with agricultural goals.