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Investigation Na+-Ca2+ interaction on biomass and enzymes activity of peroxidase and polyphenol oxidase in leaf of barley (Hordeum vulgare L.)
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S. Jahani *   , M. Lahouti , M. Jahani |
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Abstract: (3920 Views) |
| Salinity tension is a growing problem in agricultural soils. In order to investigate the interaction of Na+-Ca2+ on biomass and antioxidant enzymes activity of peroxidase and polyphenol oxidase in leaf of barley, this research was conducted as a completely randomized design with three replications in greenhouse conditions (temprature 25°C, 35 percentage relative humidity and 16 hours lighting photoperiod). The seedlings were treated by levels zero, 50, 100 and 150 mM of NaCl and with levels zero, 6 and 10 mM of CaCl2 one week after planting seeds in soil. After five weeks applying the treatment, some morphological and physiological parameters including dry weight of shoot and root and peroxidase and polyphenol oxidase enzymes activity in leaf were assessed. Results showed that salinity tension significantly decreased the dry weight of shoot and root, however, increased the peroxidase and polyphenol oxidase enzymes activity in leaf. While, adding calcium to salty environment significantly increased dry weight of shoot and root yet decreased peroxidase and polyphenol oxidase enzymes activity in leaf. Calcium decreased the harmful effect of salinity tension and the maximum ameliorative effect of calcium was observed in 6 mM CaCl2 concentration. |
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| Keywords: Peroxidase, Polyphenol oxidase, Salinity tension, Calcium chloride. |
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Full-Text [PDF 326 kb]
(2336 Downloads)
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Type of Study: Research |
Subject:
Crop Physiology
Received: 2014/08/2 | Accepted: 2014/08/2 | Published: 2014/08/2
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