Drought stress is considered an important factor in reducing the yield of wheat. For this purpose, a study was conducted on 21 wheat cultivars under different levels of cut irrigation in Varamin region. This research was carried out as split plots designed a randomized complete block with two cut irrigation and cultivars factors in 63 treatments and three replications. The main factor of irrigation treatment in three levels including; normal irrigation I0, cut irrigation in flowering stage I1, and cut irrigation in grain filling stage I2, and cultivars in 21 levels as a sub-factor including; Chamran, Star, Sirvan, Sivand, Arg, Parsi, Barat, Narin, Heidary, Ofogh, Rakhshan, Baharan, Sepahan, Aflak, Mihan, Zare, Gonhad, Orum, Saison, Gascogen and Mehregan. The results of analysis of variance showed that the simple effects of irrigation and cultivars and interactions effects on grain yield, total yield, culm leaf area index, relative leaf water content, CAT enzyme, and DT were significant at the level of 5% and 1%. Plant height and harvest index were not affected by the simple effects of irrigation treatment. Still, the simple effects of cultivars and the interaction of irrigation levels and cultivars had significant at the level of 1%. The highest plant height was obtained from normal irrigation treatment and Sepahan cultivar (113.7 Cm); grain yield was obtained from normal irrigation treatment and Rakhshan cultivar (10276.4 Kg.ha); total yield was assigned of normal irrigation treatment and Sivand cultivar with average (43892.8 Kg.ha). Catalase antioxidant enzyme was achieved from cut irrigation at the flowering stage and Sivand cultivar (54.36 U / mg protein) treatment and DT was observed from cut irrigation at the flowering stage and Gasogen cultivar treatment (21. 83 ηmol/mgprotein), The lowest plant height (76.4 Cm), grain yield (1493.7 Kg.ha), total yield (10543.9 Kg.ha) were assigned cut irrigation at flowering stage and Ofogh cultivar treatment. The lowest antioxidant enzyme CAT was obtained the normal irrigation in Sirvan cultivar treatment with average (12.95 U/mgprotein) and the biomarker DT was observed in the normal irrigation in Rakhshan cultivar treatment with average of 9.11 (ηmol/mgprotein). According to the results; Rakhshan, Sivand, and Heydari cultivars can be recommended for planting in Varamin region.