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Effect of Supplementary Irrigation and Bio fertilizers on Malondialdehyde and Anthocyanins Content and Chlorophyll Fluorescence Indices of Rainfed Barley
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Neda Ebadi   , Raouf Seyed Sharifi * , Mohammad Sedghi |
| Professor of Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran. |
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Abstract: (1435 Views) |
In order to investigate the effect of supplementary irrigation and application of mycorrhiza and Azotobacter on yield, malondialdehyde and anthocyanins content and variations of chlorophyll fluorescence of barley under rainfed conditions, an experiment as factorial was conducted based on randomized complete blocks design with three replications in 2017. Factors under investigation included irrigation at three levels [no irrigation or rainfed cultivation (I1), supplementary irrigation at 50 percent of spike emergence stage (I2), supplementary irrigation at 50 percent of spike emergence stage (I3) and biofertilizers at four levels (control or no fertilizer application) (Q1), application of mycorrhiza (Q2), Azotobacter (Q3), combined application of Azotobacter and mycorrhiza (Q4)). The results showed that the effect of supplementary irrigation and application of biofertilizers were significant on grain yield, anthocyanins and malondialdehyde content, chlorophyll index, electrical conductivity and relative water content of flag leaves, minimum fluorescence and variable fluorescence. Supplementary irrigation at booting stage and both application of mycorrhiza and Azotobacter decreased electrical conductivity (34.2%) and malondialdehyde content (157%), but quantum yield (122%), chlorophyll index (98%), anthocyanins (82%) and relative water content of flag leaf (58.7%) increased compared to non-application of bio fertilizers under rainfed conditions. The highest grain yield (268.2 gram per square meters) was obtained at supplementary irrigation at booting stage with both application of mycorrhiza and Azotobacter, which increased grain yield 29.87 percent in comparison with no application of bio fertilizers under rainfed conditions.
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| Keywords: Quantum yield, Water limitation and Relative water content. |
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Full-Text [PDF 1286 kb]
(1812 Downloads)
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Type of Study: Applicable |
Subject:
Crop Physiology
Received: 2021/02/25 | Accepted: 2021/03/30 | Published: 2021/03/30
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