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Showing 7 results for Malondialdehyde
A.r. Pazoki,
Volume 7, Issue 28 (3-2016)
Abstract
In order to investigate the effect of water deficit tension and zeolit application on the amount of damage biomarkers, relative content of leaf water, electrolytes leakage and chlorophyll of rapeseed (Brassica napus L.) Okapi cultivar, the present experiment was done as split plot based on based on randomized complete blocks design with four replications during fall 2010-11 at Islamic Azad University field research , Yadegar-e-Imam Khomein (RAH) Shahr-e-Rey Branch. Irrigation at three levels (irrigation based on 80, 130 and 180 millimeter evaporation from class A evaporation pan) as main plot, zeolite consumption in three levels (no consumption, 6 and 12 tons per hectare consumption) as sub-plot were considered. The results of experiment indicated that all traits tested were significantly different regarding to irrigation levels and zeolite application and the effect of experimented treatments interaction was significant only on electrolytes leakage and chlorophylls content. The mean comparison of main effect of irrigation demonstrated that applying irrigation based on 180 millimeter evaporation from class A evaporation pan, established the highest amount of Malondialdehyde (2.54 nanomoll per milligram protein), Dihydroxyguanosin (17.60 nanomoll per milligram protein), Dityrosine (27.02 nanomoll per milligram Protein) and electrolytes leakage (1.55 millizimence per cm) and lowest relative leaf water content (0/61 percent) and total chlorophyll (4.32 milligram per liter). In severe drought tension conditions 180 millimeter, using 12 tons per hectare zeolit consumption by the improvement of plant moisture status led to prevent drought tension harm effect and reducing leakage electrolytes to 0.99 millizimence per cm and chlorophyll a+b to 4.75 milligram per liter.
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Volume 10, Issue 39 (12-2018)
Abstract
This research was carried out in order to investigate the reaction of canola cultivars to salinity tension in a factorial experiment based on randomized complete blocks design with four replications in the research farm of Islamic Azad University, Shoushtar, during 2016-17 cropping year. In the present experiment the reaction of four canola cultivars (Hyola 320, Hyola 330, Option 500 and Sarigol) in vegetative growth stages was investigated in four salinity levels zero(control), 50, 100 and 150 mmol NaCl). The results showed the highest and lowest shoot dry weights were seen at the highest level of salinity tension in Hyola 320 (2.8 g) and Option 500 (1.5 g) and Sarigol bushes cultivars were eliminated at this salinity level. Salinity tension significantly reduced photosynthesis and chlorophyll a and b concentrations of canola cultivars, however at 150 mmol NaCl salinity level, the highest photosynthesis was observed in Hyola 320 and Heyola 330 (9.3 and 8.9 μmol Carbon dioxide per square meter of leaf area per second). Salinity tension reduced sucrose synthase enzyme activity in canola cultivars leaves, while cell membrane degradation in response to salt tension increased and the highest concentration of malondialdehyde was observed in salinity level of 150 mmol NaCl in Option 500 cultivar (0.051 nanomol per gram leaf). The results showed that Hyola 320 and Heulla 330 cultivars had more stability in cell membrane stability, photosynthesis, antioxidant enzymes activity and sucrose synthase enzyme activity compared to Option 500 and Sarigol cultivars.
Kobra Mahdavian,
Volume 11, Issue 44 (12-2019)
Abstract
Ultraviolet is divided into three strips with UV-A (320-390nm), UV-B (280-320nm) and UV-C (254-280nm) wavelengths and has adverse effects on the growth of a number of plants. Meanwhile, salicylic acids are a group of plant growth regulators that have unique biological effects on plants growth and development. Therefore, the aim of the present research was to investigate the application of salicylic acid to reduce the ultraviolet radiation tension of corn on germination, growth of shoot and root, fresh and dry weight, the amount of reducing sugars, malondialdehyde and other aldehydes. Maize plants treated with 1.5 milli Molar salicylic acid were then treated for a period of one week with UV-A, UV-B, UV-C in a completely randomized design. This investigation showed that salicylic acid significantly increased germination of maize at 5 percent probability level. The results of these researches showed that UV-B and UV-C significantly reduced the growth of shoot, root, fresh and dry weight, whereas in the pre-treated plants with salicylic acid this decrease was significantly moderated to the five percent probability level. It was also observed that UV-A had no significant effect on growth of shoot, root, fresh and dry weight. The amount of malondialdehyde and other aldehydes in plants treated with UV-B and UV-C significantly increased at a probability level of 1 percent, indicating the tension caused by UV-B and UV-C rays on the plant and the activation of defense mechanisms in these conditions. Significant decrease in the amount of leaf reductive sugars at 1 percent probability indicates radiation damage on photosynthetic apparatus. On the other hand, a significant increase in the amount of reducing sugars at the 1 percent probability level indicates the role of salicylic acid in increasing the resistance of this plant to UV-B and UV-C radiation. Considering the above, it can be concluded that the application of salicylic acid protects the plant against ultraviolet radiation.
Hamed Narimani, Raouf Seyed Sharifi, Fatemeh Aghaei,
Volume 12, Issue 47 (8-2020)
Abstract
In order to investigate the effect of methanol on the activity of antioxidant enzymes, some compatible osmolytes and biochemical traits of wheat under cut off irrigation conditions, the present experiment as factorial was conducted based on randomized complete blocks design with three replications at the research farm of Faculty of Agriculture and Natural Resources of University of Mohaghegh Ardabili, during 2015-2016 cropping year. The investigated factors were included irrigation levels (full irrigation as control, cut off irrigation in 50 percent of spike stage and cut off irrigation in 50 percent of the booting stage (pregnancy) as mild and sever water restriction, respectively; according to codes 55 and 43 of the BBCH scale) and methanol foliar application at four levels (foliar application with water as control and foliar application 10, 20 and 30 percent by volume of methanol). The results showed that foliar application of 30 percent by volume of methanol under full irrigation conditions increased 42.83, 36.01, 29.69, 57.93 and 49.98 percent of chlorophyll b content, total chlorophyll, carotenoids, protein percentage and grain yield and decreased hydrogen peroxide and malondialdehyde content 75.58 and 63.77 percent, respectively; in comparison with non-foliar application under cut off irrigation at the booting stage. Foliar application of 30 percent by volume of methanol under cut off irrigation conditions in booting stage increased proline and soluble sugars content (125.42 and 91.93 percent, respectively) in comparison with non-foliar application of methanol under full irrigation conditions. Also, foliar application of 30 percent by volume of methanol under full irrigation conditions decreased the activity of antioxidant enzymes (catalase, peroxidase and polyphenol oxidase). It seems that foliar application of 30 percent by volume of methanol can increase grain yield of wheat under cut off irrigation conditions due to improving biochemical traits.
Davar Molazem, Ali Bashirzadeh, Jafar Azimi,
Volume 13, Issue 51 (12-2021)
Abstract
To investigate the effect of foliar application of salicylic acid concentrations (zero, one and two milli Molar) on biochemical and physiological characteristics of three maize genotypes SC704, NS640 and SC580 in salinity tension (zero, 50 and 100 milli Molar NaCl) a pot experiment was carried out by factorial split plot in randomized complete blocks design with three replications at the research station of Islamic Azad University, Ardabil Branch in 2014-2015 cropping year. The results showed that most traits had significant differences in different salinity and salicylic acid levels. Comparison of means showed that salinity tension increased proline, superoxide dismutase, malondialdehyde, sodium content and electrolyte leakage to 70.49, 145, 58.99, 92.86 and 12.6 percent, respectively, while carotenoids, membrane stability index and potassium decreased by 36.41, 20.32 and 39.68 percent, respectively. Foliar application of salicylic acid increased superoxide dismutase enzyme by 24 percent compared to the control, but there was no significant difference between one or two milli Molar. NS64 variety showed the highest superoxide dismutase enzyme. Maize genotypes under salinity tension had higher malondialdehyde content; however, the lowest amount of malondialdehyde (6,538 mol units per gram of wet weight) was observed in non-saline conditions and the highest level of salicylic acid. Foliar application of salicylic acid moderated the negative effect of salinity tension on the traits and increased carotenoids, potassium and membrane stability-index and decreased malondialdehyde content and electrolyte leakage. SC704 genotype with the highest amount of proline, malondialdehyde and potassium showed the highest index of membrane stability.
Mohammad Mahdi Motiei, Alireza Sirousmehr, Isa Khamari, Ahmad Naderi,
Volume 13, Issue 51 (12-2021)
Abstract
One of the most important farm managements to achieve optimal plant growth conditions and proper yield is to provide sufficient water so that the plant does not suffer from moisture tension in the critical stages of growth. The present study was conducted as split plots in a randomized complete block design with 4 replications in Ahvaz Agricultural Research Center during 2016-2017. Irrigation regimes after evaporation of 50, 70 and 90 millimeter from class A evaporation pan were placed in the main plots and six cultivars of Progress, Farrokh, Barzegar, Record, Azargol and Lakomka were placed in the sub-plots. The results showed that plant height, head diameter, one-thousand seed weight, oil percentage, grain yield, biological yield, photosynthetic pigments decreased significantly with increasing irrigation regime from 50 to 90 millimeter evaporation from class A evaporation pan and proline and Malondialdehyde traits increased.Also, the tripartite effects of year, irrigation regime and cultivar for one-thousand grain weight, grain yield and proline were significant. The highest one-thousand seed weight (80.3 gram) and grain yield (2529 kilogram per hectare) was obtained from the treatment combination of 50 millimeter evaporation from the evaporation pan and the Record cultivar during two years of experiment and the highest proline concentration (20.8 milligram per gram fresh leaf weight) in the treatment composition of 90 millimeter evaporation from the evaporation pan and Record cultivar in two years. Therefore, according to the results for Ahvaz region, Record cultivar and irrigation of 50 millimeter from the evaporation pan is recommended.
Fatemeh Basiri Rad, Raouf Seyed Sharifi, Khoshnaz Payandeh, Sara Mohammadi Kale Sarlou,
Volume 16, Issue 61 (5-2024)
Abstract
Water limitation is one of the most important abiotic stresses that can decrease growth and yield of rye (Secale cereal L.). Several strategies have been suggested in order to improve yield of rye under water limitation conditiond, among them application of mycorrhiza, nanoparticles of iron and silicon play a key role in yield improvement. In this regard, in order to study the effects of mycorrhiza, nanoparticles of iron and silicon on some physiological and biochemical traits of rye (Secale cereal L.) under withholding irrigation conditions, an experimental as factorial was conducted based on randomized complete block design with three replications in research greenhouse of Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili during 2022. Experimental factors were included irrigation in three levels (full irrigation as control, withholding irrigation at 50% of heading and booting stages), mycorrhiza application at two levels (no application as control, application of mycorrhiza), nano iron-silicon oxide foliar application at four levels (foliar application with water as control, nano iron oxide foliar application, nano silicon, both application nano iron-silicon oxide). The results showed that withholding irrigation decreased relative water content and stomatal conductance in all of sampling stages, but application of nanoparticles and mycorrhiza were able to compensate of part of this reduction. Also, both application nanoparticle and mycorrhiza under withholding irrigation at booting stage decreased malondialdehyde and hydrogen peroxide contents (58.87 and 36.04% respectively) in comparison with no application of nanoparticle and mycorrhiza in the same irrigation level. But, it increased the activity of catalase, peroxidase and polyphenol oxidase enzymes (34.67, 32.14 and 32.18% respectively), proline and soluble sugars contents (38.01 and 28.33% respectively) in comparison with no application of mycorrhiza and nano iron-silicon oxide in the same irrigation level. Maximum of grain yield (2.76 g.per plant) was obtained at both application nanoparticle and mycorrhiza in full irrigation, which increased grain yield of 45.2% in comparison with no application of mycorrhiza and nano iron-silicon oxide under withholding irrigation at booting stages. Based on the results of this study, it can be suggested that under withholding irrigation conditions, application of nanoparticles and mycorrhiza can increase grain yield of rye by improving the physiological and biochemical traits.
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