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Showing 3 results for Superoxide Dismutase
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Volume 8, Issue 31 (12-2016)
Abstract
This research was carried out in order to biomolecular investigate drought tension tolerance in two wheat cultivars inoculated with plant growth promoting rhizobacteria (PGPR) in the research greenhouse of Islamic Azad University, Karaj Branch in 2013-14 cropping year. The experiment design was in the split factorial arrangement based on a randomized complete blocks design with three replications. The main plot included two levels of desirable irrigation and drought tension (deficit irrigation in the pollination stage until the end of full maturity) and sub plots consisted the PGPR bacteria at four levels: 1) inoculation seed with Azotobacter chroococcum 2) inoculation seed with Azospirillum lipoferum 3) simultaneous use of both bacteria Azotobacter + Azospirillum 4) non-application of bacteria (control) and two bread wheat cultivars consisted of Sirvan and Marvdasht. Experiment traits included the activity of superoxide dismutase antioxidant enzyme (SOD), the activity of biochemical biomarker Malondialdehyde (MDA) amount of chlorophyll a, b and a + b. Results of the experiment showed that simple effect of irrigation, cultivar and bacteria treatments was significant on all the studied traits (01 / 0≥P). The activity of superoxide dismutase enzyme (SOD) in drought tension treatment was higher than full irrigation (normal). Sirwan and Marvdasht cultivars with 3.6 and 2.7 units per gram of protein, showed the higher and lower SOD enzyme activity, respectively. Sirvan cultivar compared to Marvdasht cultivar had less amount of biomarker MDA activity (27.424 milligram on gram fresh weight of tissue) and the higher content of chlorophyll a, b and a+b with 3.255, 1.578 and 4.811 (milligram on gram fresh weight of tissue), respectively.
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Volume 9, Issue 34 (9-2017)
Abstract
In order to investigate the effect of seed priming with salicylic acid and water deficit tension on enzyme activity and yield of grain corn, the present experiment was conducted as split plot based on randomized completely blocks design with three replications at the Agricultural Research Station of Islamic Azad University, Tabriz Branch in 2015. The Main factor was consisted of two levels of normal irrigation (without tension) and water deficit application based on 70 and 110 millimeter evaporation from evaporation pan class A, respectively after from 8-9 leaf stage of corn and sub-factor consisted of three levels of corn seed priming with salicylic acid concentrations of 100 and 200 ppm with the control (no priming) in pre-planting stage. According to the results of the experiment, superoxide dismutase enzyme activity increased by 27 percent in maize leaf due to occurrence of water deficit tension and seed priming with salicylic acid concentrations of 100 and 200 ppm increased superoxide dismutase activity by 32 and 42 percent, respectively. Corn grain yield by applying water deficit tension with increasing water irrigation period from 70 to 110 mill meter evaporation from pan decreased as 110 grams per square meter from 8-9 leaf stage onwards, and seed priming with salicylic acid concentrations of 100 and 200 ppm increased corn grain yield up to an average of 13.50 percent and no significant difference was observed in the grain yield between two concentrations. Therefore, due to the lack of significant difference in terms of studied traits and especially grain yield between two levels of concentration, it seems that seed priming with 100 ppm salicylic acid concentration in order to increase corn grain yield in both cases, normal irrigation and water deficit tension have been effective.
Davar Molazem, Ali Bashirzadeh, Jafar Azimi,
Volume 13, Issue 51 (12-2021)
Abstract
To investigate the effect of foliar application of salicylic acid concentrations (zero, one and two milli Molar) on biochemical and physiological characteristics of three maize genotypes SC704, NS640 and SC580 in salinity tension (zero, 50 and 100 milli Molar NaCl) a pot experiment was carried out by factorial split plot in randomized complete blocks design with three replications at the research station of Islamic Azad University, Ardabil Branch in 2014-2015 cropping year. The results showed that most traits had significant differences in different salinity and salicylic acid levels. Comparison of means showed that salinity tension increased proline, superoxide dismutase, malondialdehyde, sodium content and electrolyte leakage to 70.49, 145, 58.99, 92.86 and 12.6 percent, respectively, while carotenoids, membrane stability index and potassium decreased by 36.41, 20.32 and 39.68 percent, respectively. Foliar application of salicylic acid increased superoxide dismutase enzyme by 24 percent compared to the control, but there was no significant difference between one or two milli Molar. NS64 variety showed the highest superoxide dismutase enzyme. Maize genotypes under salinity tension had higher malondialdehyde content; however, the lowest amount of malondialdehyde (6,538 mol units per gram of wet weight) was observed in non-saline conditions and the highest level of salicylic acid. Foliar application of salicylic acid moderated the negative effect of salinity tension on the traits and increased carotenoids, potassium and membrane stability-index and decreased malondialdehyde content and electrolyte leakage. SC704 genotype with the highest amount of proline, malondialdehyde and potassium showed the highest index of membrane stability.
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