In order to investigate the effect of nip and drought tension on chlorophyll index, relative water content, electrolyte leakage and grain yield of maize single cross 400 under different levels of salicylic acid, a field experiment carried out in split split plots in randomized complete block design with three replications in the greenhouse and Agricultural Research Station of Ferdowsi University of Mashhad during the cropping year of 2014. In the present experiment the main factor, cold tension at two levels (non- nip tension and nip tension), sub-factor included three levels of drought tension (irrigation equivalent to 100 percent plant water requirement (non-tension, control)), irrigation equivalent to 75 percent of plant water requirement and irrigation equivalent to 50 percent plant water requirement) and sub sub factor foliar application salicylic acid (non-foliar application (control), foliar application 200 micro molar and 400 micro molar foliar application) was considered. The obtained results indicate chlorophyll meter index, relative water content and electrolyte leakage are influenced under drought tension conditions; as water deficiency tension, chlorophyll index decreased relative water content and electrolyte leakage, increased grain yield. Also, salicylic acid foliar application improved the chlorophyll index, leaf relative water content, increasing electrolyte leakage and grain yield. Nip treatment interaction on drought tension using 200 micro molar foliar application of salicylic acid improved the chlorophyll index. According to the results, using the appropriate concentration of salicylic acid are recommended for improving environmental tensions such as drought and nip to achieve the optimal performance.