Considering the importance of agricultural issues in adjacent lands to normal saline watersheds and its effect on plant growth and production, the present study was carried out in order to investigate the interaction effect of arbuscular mycorrhizal fungi and plant growth regulator of brassinolide on the possible reduction of adverse effect of salinity on wheat Pishtaz cultivar. The present research in a factorial randomized complete blocks design was conducted with four replications. Thus, mycorrhizal plants with Glomus mice (after ensuring mycorrhizal roots) and non-mycorrhizal 14 days by 24-epibrassinolide with zero and five micro molar concentration were foliar spray three times every other day. Then, they were irrigated by saline water originated from Urmia Lake and the electrical conductivity (EC) zero and 15 ds m per meter one time every three days. Finally, the 26-day-old plants were harvested and transported to the laboratory in order to perform some physiological measures. Findings showed an improvement in leaf area and reducing membrane leakage in separate fungal and brassinolide treatments. While the interaction effect of these two treatments only in the leaf area at the level of P< 0/05 was significant. Also, the effect of significant synergistic interaction between the two treatments by the higher antioxidant activity including antioxidant of superoxide dismutase and the amount of leaf anthocyanin and also the percentage scavenging of free radicals (DPPH) was observed.