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The comparison of the effect of soil cobalt concentration on chlorophyll a.b and totall chlorophill density in two cultivars of pinto bean Khomein and Talash
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M. Sharafi *   , A. Ranjbar , H. Beigi Harchegani |
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Abstract: (4425 Views) |
| In order to investigate the effect of soil cobalt concentrations on chlorophyll a,b and total chlorophill density in pinto bean,a pot experiment was conducted in Shahrekord University's greenhouse,separatly for two pinta beans cultivars Khomein and Talash in cropping year 2010-2011. The experiment included five treatments (0,20,70,150,and 220 mg of cobalt chloride per kg of dry soil) and four replicates arranged in a completely randomized design. After physiological maturity of plants,chlorophylls a and b were measured and total chlorophyll in middle leaves of each pod was calculated as sum of chlorophylls a and b. Results showed a tendency to increase (up to soil CoCl2 concentrations of 20 mg/kg) and then a decrease in both chlorophylla.b and totall densities (at soil CoCl2 concentrations more than 70 mg/kg). Totally,Soil CoCl2 concentrations of 150 and 220 mg/kg significantly decreased the chlorophyll densities compared to control (P<0.05). Despite the negative and significant effect of high soil cobalt,more than 100mg/kg CoCl2, it seems that low soil cobalt(lower than 20mg) for Khomein cultivar and lower than 70mg Co for Talash,may have positive effect on chlorophyll densities in pinto bean leaves. The tolerance threshold for chlorophylls a,b and total chlorophyll were estimated to be approximately 60,30 and 40 mg of CoCl2 per kg of dry soil,respectively,for Khomein pinto bean.For Talash cultivar,the tolerance threshold for chlorophylls b and total was obtained 90 and 55 mgCoCl2 on kg soil. The synthesis of chlorophyll b in pinto beans leaf,in Khomein and Talash cultivars, is more sensitive to high soil cobalt conditions than chlorophylla. |
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| Keywords: Cobalt, Bean, Chlorophyll. |
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Full-Text [PDF 153 kb]
(2401 Downloads)
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Type of Study: Research |
Subject:
Crop Physiology
Received: 2014/05/18 | Accepted: 2014/05/18 | Published: 2014/05/18
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