fa اثرات تنش سرما بر بعضی آنزیم‌های آنتی اکسیدانت و صفات فیزیولوژیک یولاف زراعی در گلخانه فیزیولوژی گیاهان زراعی Crop Physiology كاربردي Applicable <div style="text-align: justify;">&nbsp;<span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">به‌منظور ارزیابی تنوع ژنوتیپ‌های یولاف زراعی </span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">(</span></span></span></b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Avena sativa </span></span></span></i></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">L.</span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">)</span></span></span></b><b><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> در پاسخ به تنش سرما، 21 ژنوتیپ یولاف در سال 96-1395 در دانشکده علوم و مهندسی کشاورزی دانشگاه رازی کرمانشاه تحت شرایط کنترل شده در گلخانه مورد بررسی قرار گرفتند. دو آزمایش جداگانه جهت </span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">تعیین</span></span></span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">دمای</span></span></span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">50 درصد کشندگی گیاه </span></span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">(</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">LT50</span></span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">)</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black"> و تأثیر تنش کوتاه مدت سرما بر خصوصیات فیزیولوژیک و آنزیم‌های آنتی‌اکسیدانت، در قالب طرح کاملاً تصادفی ا&shy;نجام شد</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">. تجزیه واریانس صفت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">LT50</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> نشان داد که تنوع ژنتیکی معنی‌داری بین ژنوتیپ‌های بررسی شده از نظر این صفت وجود دارد.</span></span></span></b><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">نتایج مقایسه میانگین صفت </span></span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">LT50</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black"> به روش دانکن نشان داد که ژنوتیپ</span></span></span></span></b><b><span dir="LTR" lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">&lrm;</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">های (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Ozark</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">)، (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">UFRGSP 948886</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">)، (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">UPF775456</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">)، (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Euro</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">)، (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">GA Mitchell</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">)، (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Mortlock</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">)، (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">OH1022</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">)، (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Brusher</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">)، (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Wallaroo</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">) و (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Kalott</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">) بیشترین مقدار </span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">LT50</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black"> را دارا بوده و می‌توان آن‌ها را به‌عنوان ژنوتیپ&lrm;های متحمل به سرما در نظر گرفت و ژنوتیپ‌های (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Quoll</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">) و (</span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Nasta</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">) کمترین مقدار را دارا بودند.</span></span></span></span></b> <b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">تجزیه واریانس نشان داد اثر تیمار دمایی بر صفات میزان کاروتنوئید، نشت یونی، کاتالاز و پراکسیداز معنی&lrm;دار بود</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" style="font-family:" zar="">.</span></span></span><b> </b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">همبستگی مثبت و معنی‌داری بین آنزیم کاتالاز با محتوای کلروفیل و بین آنزیم پراکسیداز با میزان نشت یونی مشاهده شد. </span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">ژنوتیپ‌های </span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Ozark</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">UFRGS 948886</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black"> در تجزیه خوشه‌ای در گروهی با حداکثر میانگین </span></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:black">LT50</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">، محتوای کلروفیل، محتوای کاروتنوئید، آنزیم‌های کاتالاز، پراکسیداز و حداقل میانگین میزان نشت یونی قرار گرفتند. بنابراین می‌توان گفت ژنوتیپ‌های این گروه وضعیت بهتری از نظر تحمل به سرما نسبت به سایر ژنوتیپ‌ها دارند.</span></span></span></span></b><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></b></span></span></span></span></span></div> <div style="text-align: justify;">&nbsp;<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">In order to evaluate the diversity of oat genotypes (<i>Avena sativa</i> L.) in response to cold stress, 21 oat genotypes were studied in the Faculty of Agricultural Sciences and Engineering of Razi University, Kermanshah in 2015-2016 under controlled conditions in the greenhouse. Two separate experiments were conducted to determine the 50% plant lethality temperature (LT50) and the effect of short-term cold stress on physiological characteristics and antioxidant enzymes, in the form of a completely randomized design. Variance analysis of LT50 showed that there is a significant genetic variation between the investigated genotypes in terms of LT50 level. The average of LT50 trait showed that the genotypes (Ozark), (UFRGSP 948886), (UPF775456), (Euro), (GA Mitchell), (Mortlock), (OH1022), (Brusher), (Wallaroo) and (Kalott) had the highest LT50 value and can be considered as cold tolerant genotypes, and (Quoll) and (Nasta) genotypes had the lowest values. A positive and significant correlation was observed between the catalase enzyme and chlorophyll content and between the peroxidase enzyme and ion leakage rate. The Ozark and UFRGS 948886 genotypes were placed in the group with the maximum mean LT50, chlorophyll content, carotenoid content, catalase enzyme, peroxidase enzyme and minimum ion leakage rate in the cluster analysis. Therefore, it could be stated that the genotypes of this group have better conditions in terms of cold tolerance than other genotypes.</span></span></b></span></span></span></span></div> آنزیم‎های آنتی اکسیدانت, تنش سرما, یولاف, LT50 Antioxidant Enzymes, Cold Stress, Oat, LT50 113 128 http://cpj.ahvaz.iau.ir/browse.php?a_code=A-10-1372-2&slc_lang=fa&sid=1 Sohbat Bahraminejad صحبت بهرامی نژاد sohbah72@hotmail.com 3369890909 100319475328460010568 Yes Razi University دانشگاه رازی فاطمه رومیانی fateme_roumiani@yahoo.com 3240479621 100319475328460010569 No Razi University دانشگاه رازی لیلا زارعی zare_l@yahoo.com 3308978296 100319475328460010570 No Razi University دانشگاه رازی