fa اثر پرایمینگ بر خصوصیات جوانه‌زنی بذر و گیاهچه برنج رقم عنبر بو، تحت تنش کم آبی فیزیولوژی گیاهان زراعی Crop Physiology كاربردي Applicable <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">این آزمایش به&shy;منظور ارزیابی اثر صفات مورفولوژیکی و بیوشیمیایی بر تحمل به تنش کم&shy;آبی در گیاهچه&shy;های برنج، </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">به&shy;صورت فاکتوریل </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">دو عاملی </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در قالب طرح کاملاٌ تصادفی</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> چهار </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تکرار </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آزمایشگاه تکنولوژی بذر دانشگاه شاهد تهران در سال 1397</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">انجام شد.</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عامل اول </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">سطوح مختلف تنش کم آبی شامل صفر، 5/0- و 1- مگاپاسکال با پلی اتیلن گلایکول 6000 </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">عامل</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">دوم</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> سطوح مختلف </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">پرایمینگ</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">شامل،</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">شاهد</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> (</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بذور</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بدون</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">هیدروپرایم</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">)، پیش تیمار آبی (هیدروپرایمینگ)، نیترات پتاسیم 5/0 درصد،</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> سویه</span></span></strong><strong><span dir="LTR"><span style="font-size:10.0pt;">&shy;</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">های باکتری سودوموناس فلورسنس (</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PF</span></span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">)، سویه باکتری باسیلوس سوبتلیس </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:9.0pt;">(</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">BS</span></span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:9.0pt;">)</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> و اثر توأم دو باکتری </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">PF</span></span></span></strong><strong><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">&times;</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:9.0pt;">BS</span></span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">به مدت 24 ساعت در دمای 15 درجه سانتی&shy;گراد قرار گرفت. </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">با</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">توجه</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">به</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نتایج</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آزمایش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">پرایمینگ</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تنش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کم&shy;آبی</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">اثر معنی</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&shy;</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">داری</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بر</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">صفات</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">مورد</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">پژوهش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">داشتند</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">. </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بذور</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">برنج</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">دارای</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">درصد</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">جوانه</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&shy;</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">زنی</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بالایی</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بودند</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> که بیشترین</span></span></strong><strong><span dir="LTR"><span style="font-size:10.0pt;">&shy;</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آن در پیش تیمار </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نیترات پتاسیم، در دو سطح شاهد و 5/0- مگاپاسکال مشاهده شد </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کم</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&shy;</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ترین</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آن</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> 80 </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">درصد</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تلقیح</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">همزمان</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">باکتری</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&shy;</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ها</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">بود</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">. </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کاهش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">خصوصیات</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">جوانه</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&shy;</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">زنی،</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">پارامترهای</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">رشد،</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">محتوای</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">رطوبت</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نسبی</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">با</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">افزایش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تنش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">رخ</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">داد</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">هم</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">چ</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">نین</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">افزایش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">رنگدانه</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">&shy;</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">های</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">فتوسنتزی،</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">محتوای</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آنتوسیانین در تیمار باکتری سوبتلیس با میانگین (56/12) میلی&shy;گرم بر گرم وزن تر برگ مربوط به تنش 1- مگاپاسکال،</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">پرولین در تیمار هیدروپرایمینگ عدم تنش با میانگین (85/8) میلی&shy;گرم بر گرم وزن تر برگ مربوط به تنش 1- مگاپاسکال</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">همچنین</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">فعالیت آنزیم</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">سوپراکسید</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">دسموتاز</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">در تیمار نیترات پتاسیم و باکتری فلورسنس به ترتیب 72/1342 و 17/1355 درصد مربوط به تنش 1- مگاپاسکال در</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">افزایش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تنش</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کم&shy;آبی به&shy;دست</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">آمد</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">.</span></span></strong> <strong>This experiment was conducted to evaluate the effect of morphological and biochemical traits on dehydration tension tolerance in rice seedlings in a two -factorial factorial design in a completely randomized design with four replications in Seed Technology Laboratory of Shahed University of Tehran, in 2018. The first factor was different levels of water deficit tension including 0, -0.5 and -1 Mega Pascal with polyethylene glycol 6000 and the second factor was different levels of priming including control (seeds without hydro prime), water pre-treatment (Hydro priming), potassium nitrate 0.5 percent, Pseudomonas fluorescens (PF) strains, Bacillus subtilis (BS) bacteria strain and the combined effect of two bacteria PF &times; BS for 24 hours at 15</strong><strong>&ordm;</strong><strong>C. Considering the results of priming experiment and water deficit tension, there was a significant effect on the studied traits. Rice seeds had a high germination percentage, most of which was observed in potassium nitrate pre-treatment at two levels of control and -0.5 Mega Pascal and the lowest of which was at 80 percent in simultaneous bacterial inoculation. Rice seeds had a high germination percentage, the highest of which was observed in the pre-treatment of potassium nitrate, at control and -0.5 Mega Pascal levels and the lowest was at 80 percent in simultaneous bacterial inoculation. Reducing germination characteristics, growth parameters, relative humidity content happened with increasing tension, as well as enhancing photosynthetic pigmentation, anthocyanin content in Substile bacteria treatment with mean (12.56) mg/g fresh weight of leaf relative to -1 Mega Pascal, proline in hydroperiming treatment with non-tension by average (8.85) mg/g fresh weight of leaves related to -1 Mega Pascal and also superoxide dismutase enzyme activity in potassium nitrate and fluorescence bacteria treatments 1342.72 and 1355.17 percent related to -1 Mega Pascal tension in increasing water deficit tension.</strong> پلی‌اتیلن گلایکول, پیش تیمار, درصد جوانه‌زنی, رنگدانه و برنج. Polyethylene glycol, Germination percentage, Pigment and Rice. 5 21 http://cpj.ahvaz.iau.ir/browse.php?a_code=A-10-1-202&slc_lang=fa&sid=1 Seyed Ali Latifi سید علی لطیفی 10031947532846006263 10031947532846006263 No Heshmat Omidi حشمت امیدی 10031947532846006264 10031947532846006264 Yes